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|
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| System
requirements: |
Windows
XP, 2000, NT4, Me, 98
300MHz PII, 128MB RAM suggested
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| Current
version:
|
V2.6.49
(see
revision history)
|
| Notes: |
If
you currently have V2.4.56 then
please uninistall it before
installing this version |
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|
|
|
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| Price
per key (US dollars) |
| Number of keys in
order |
1 |
2 |
3+ |
| Academic licence |
$1995 |
$1495 |
$1295 |
| Commercial licence |
$2995 |
$2395 |
$1995 |
|
|
|
Overview
Koadarray is fully automatic array image analysis
software which can process single or multiple array images entirely unattended. It automatically finds the spot locations within each image and accurately quantifies the spot intensity data. Koadarray is also very fast, a 5000 spot image may be fully analysed and quantified in 30 seconds with an average computer.
Koadarray has been developed primarily for fluorescent microarray image
analysis, but may also be found to work in radioactive or macroarray applications. It has also been found to work with fluorescent microplate images.
Koadarray is extremely easy to use, with simple and intuitive controls. Also, unlike other array image analysis programs that require you to define numerous grid details such as spot size and row/column spacing, the unique spot finding algorithms used by Koadarray mean that you only need to input the number of blocks and spots.
Image analysis
A range of different image file formats can be opened, either dual colour or monochrome, and almost any rectangular spot and block layout can be analysed. Koadarray can analyse most images successfully in spite of image defects. It provides helpful error messages if it encounters difficulties or if the found spot positions do not appear to correspond with others in the image.
If the automatic spot finding is unable to determine some spot positions, as may be the case in images with many missing or indistinct spots, the positions of spots or blocks can be manually adjusted. A number of user-settable spot finding parameters are provided that can be altered if a problem image is encountered. Optimised default values that have been found to work over a wide range of images are provided for all of these parameters.
Quantification and data output
After finding the spots, Koadarray performs a sophisticated spot intensity quantification. This includes an assessment and removal of detected artefact pixels within the spot area and accurate local background subtraction. The data are then saved in a tab-delimited text
file (example), the format of which can be configured to suit specific data processing requirements or retain compatibility with
existing systems. Optionally, additional spot information may also be saved, including a spot confidence value and spot/gene IDs. The output data file also contains a log of the spot finding and quantification process, including array quality values and any errors encountered. In order to review any analysis problems after multiple image processing, a bitmap of the analysed image may also be automatically saved.
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